Loss of heterozygosity by SCRaMbLEing.
Identifieur interne : 000290 ( Main/Exploration ); précédent : 000289; suivant : 000291Loss of heterozygosity by SCRaMbLEing.
Auteurs : Yunxiang Li [République populaire de Chine] ; Yi Wu [République populaire de Chine] ; Lu Ma [République populaire de Chine] ; Zhou Guo [République populaire de Chine] ; Wenhai Xiao [République populaire de Chine] ; Yingjin Yuan [République populaire de Chine]Source :
- Science China. Life sciences [ 1869-1889 ] ; 2019.
Descripteurs français
- KwdFr :
- Aneuploïdie (MeSH), Chromosomes de champignon (génétique), Facteurs de transcription (génétique), Génome fongique (génétique), Humains (MeSH), Hétérozygote (MeSH), Instabilité du génome (MeSH), Perte d'hétérozygotie (génétique), Phénotype (MeSH), Protéines de Saccharomyces cerevisiae (génétique), Résistance microbienne aux médicaments (effets des médicaments et des substances chimiques), Résistance microbienne aux médicaments (génétique), Saccharomyces cerevisiae (génétique), Sirolimus (pharmacologie), Techniques génétiques (MeSH), Variation génétique (MeSH), Évolution moléculaire (MeSH).
- MESH :
- effets des médicaments et des substances chimiques : Résistance microbienne aux médicaments.
- génétique : Chromosomes de champignon, Facteurs de transcription, Génome fongique, Perte d'hétérozygotie, Protéines de Saccharomyces cerevisiae, Résistance microbienne aux médicaments, Saccharomyces cerevisiae.
- pharmacologie : Sirolimus.
- Aneuploïdie, Humains, Hétérozygote, Instabilité du génome, Phénotype, Techniques génétiques, Variation génétique, Évolution moléculaire.
English descriptors
- KwdEn :
- Aneuploidy (MeSH), Chromosomes, Fungal (genetics), Drug Resistance, Microbial (drug effects), Drug Resistance, Microbial (genetics), Evolution, Molecular (MeSH), Genetic Techniques (MeSH), Genetic Variation (MeSH), Genome, Fungal (genetics), Genomic Instability (MeSH), Heterozygote (MeSH), Humans (MeSH), Loss of Heterozygosity (genetics), Phenotype (MeSH), Saccharomyces cerevisiae (genetics), Saccharomyces cerevisiae Proteins (genetics), Sirolimus (pharmacology), Transcription Factors (genetics).
- MESH :
- chemical , genetics : Saccharomyces cerevisiae Proteins, Transcription Factors.
- drug effects : Drug Resistance, Microbial.
- genetics : Chromosomes, Fungal, Drug Resistance, Microbial, Genome, Fungal, Loss of Heterozygosity, Saccharomyces cerevisiae.
- chemical , pharmacology : Sirolimus.
- Aneuploidy, Evolution, Molecular, Genetic Techniques, Genetic Variation, Genomic Instability, Heterozygote, Humans, Phenotype.
Abstract
Genetic variation drives phenotypic evolution within populations. Genetic variation can be divided into different forms according to the size of genomic changes. However, study of large-scale genomic variation such as structural variation and aneuploidy is still limited and mainly based on the static, predetermined feature of individual genomes. Here, using SCRaMbLE, different levels of loss of heterozygosity (LOH) events including short-range LOH, long-range LOH and whole chromosome LOH were detected in evolved strains. By contrast, using rapid adaptive evolution, aneuploidy was detected in the adaptive strains. It was further found that deletion of gene GLN3, long-range LOH in the left arm of synthetic chromosome X, whole chromosome LOH of synthetic chromosome X, and duplication of chromosome VIII (trisomy) lead to increased rapamycin resistance in synthetic yeast. Comparative analysis of genome stability of evolved strains indicates that the aneuploid strain has a higher frequency of degeneration than the SCRaMbLEd strain. These findings enrich our understanding of genetic mechanism of rapamycin resistance in yeast, and provide valuable insights into yeast genome architecture and function.
DOI: 10.1007/s11427-019-9504-5
PubMed: 30900161
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Xiao</name><affiliation wicri:level="1"><nlm:affiliation>Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, 300072, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, 300072</wicri:regionArea><wicri:noRegion>300072</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, 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heterozygosity by SCRaMbLEing.</title><author><name sortKey="Li, Yunxiang" sort="Li, Yunxiang" uniqKey="Li Y" first="Yunxiang" last="Li">Yunxiang Li</name><affiliation wicri:level="1"><nlm:affiliation>Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, 300072, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, 300072</wicri:regionArea><wicri:noRegion>300072</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072</wicri:regionArea><wicri:noRegion>300072</wicri:noRegion></affiliation></author><author><name sortKey="Wu, Yi" sort="Wu, Yi" uniqKey="Wu Y" first="Yi" last="Wu">Yi Wu</name><affiliation wicri:level="1"><nlm:affiliation>Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, 300072, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, 300072</wicri:regionArea><wicri:noRegion>300072</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072</wicri:regionArea><wicri:noRegion>300072</wicri:noRegion></affiliation></author><author><name sortKey="Ma, Lu" sort="Ma, Lu" uniqKey="Ma L" first="Lu" last="Ma">Lu Ma</name><affiliation wicri:level="1"><nlm:affiliation>Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, 300072, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, 300072</wicri:regionArea><wicri:noRegion>300072</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072</wicri:regionArea><wicri:noRegion>300072</wicri:noRegion></affiliation></author><author><name sortKey="Guo, Zhou" sort="Guo, Zhou" uniqKey="Guo Z" first="Zhou" last="Guo">Zhou Guo</name><affiliation wicri:level="1"><nlm:affiliation>Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, 300072, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, 300072</wicri:regionArea><wicri:noRegion>300072</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072</wicri:regionArea><wicri:noRegion>300072</wicri:noRegion></affiliation></author><author><name sortKey="Xiao, Wenhai" sort="Xiao, Wenhai" uniqKey="Xiao W" first="Wenhai" last="Xiao">Wenhai Xiao</name><affiliation wicri:level="1"><nlm:affiliation>Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, 300072, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, 300072</wicri:regionArea><wicri:noRegion>300072</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072</wicri:regionArea><wicri:noRegion>300072</wicri:noRegion></affiliation></author><author><name sortKey="Yuan, Yingjin" sort="Yuan, Yingjin" uniqKey="Yuan Y" first="Yingjin" last="Yuan">Yingjin Yuan</name><affiliation wicri:level="1"><nlm:affiliation>Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, 300072, China. yjyuan@tju.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, 300072</wicri:regionArea><wicri:noRegion>300072</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China. yjyuan@tju.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072</wicri:regionArea><wicri:noRegion>300072</wicri:noRegion></affiliation></author></analytic><series><title level="j">Science China. Life sciences</title><idno type="eISSN">1869-1889</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aneuploidy (MeSH)</term><term>Chromosomes, Fungal (genetics)</term><term>Drug Resistance, Microbial (drug effects)</term><term>Drug Resistance, Microbial (genetics)</term><term>Evolution, Molecular (MeSH)</term><term>Genetic Techniques (MeSH)</term><term>Genetic Variation (MeSH)</term><term>Genome, Fungal (genetics)</term><term>Genomic Instability (MeSH)</term><term>Heterozygote (MeSH)</term><term>Humans (MeSH)</term><term>Loss of Heterozygosity (genetics)</term><term>Phenotype (MeSH)</term><term>Saccharomyces cerevisiae (genetics)</term><term>Saccharomyces cerevisiae Proteins (genetics)</term><term>Sirolimus (pharmacology)</term><term>Transcription Factors (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Aneuploïdie (MeSH)</term><term>Chromosomes de champignon (génétique)</term><term>Facteurs de transcription (génétique)</term><term>Génome fongique (génétique)</term><term>Humains (MeSH)</term><term>Hétérozygote (MeSH)</term><term>Instabilité du génome (MeSH)</term><term>Perte d'hétérozygotie (génétique)</term><term>Phénotype (MeSH)</term><term>Protéines de Saccharomyces cerevisiae (génétique)</term><term>Résistance microbienne aux médicaments (effets des médicaments et des substances chimiques)</term><term>Résistance microbienne aux médicaments (génétique)</term><term>Saccharomyces cerevisiae (génétique)</term><term>Sirolimus (pharmacologie)</term><term>Techniques génétiques (MeSH)</term><term>Variation génétique (MeSH)</term><term>Évolution moléculaire (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Saccharomyces cerevisiae Proteins</term><term>Transcription Factors</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Drug Resistance, Microbial</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Résistance microbienne aux médicaments</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Chromosomes, Fungal</term><term>Drug Resistance, Microbial</term><term>Genome, Fungal</term><term>Loss of Heterozygosity</term><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Chromosomes de champignon</term><term>Facteurs de transcription</term><term>Génome fongique</term><term>Perte d'hétérozygotie</term><term>Protéines de Saccharomyces cerevisiae</term><term>Résistance microbienne aux médicaments</term><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Sirolimus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Sirolimus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Aneuploidy</term><term>Evolution, Molecular</term><term>Genetic Techniques</term><term>Genetic Variation</term><term>Genomic Instability</term><term>Heterozygote</term><term>Humans</term><term>Phenotype</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Aneuploïdie</term><term>Humains</term><term>Hétérozygote</term><term>Instabilité du génome</term><term>Phénotype</term><term>Techniques génétiques</term><term>Variation génétique</term><term>Évolution moléculaire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Genetic variation drives phenotypic evolution within populations. Genetic variation can be divided into different forms according to the size of genomic changes. However, study of large-scale genomic variation such as structural variation and aneuploidy is still limited and mainly based on the static, predetermined feature of individual genomes. Here, using SCRaMbLE, different levels of loss of heterozygosity (LOH) events including short-range LOH, long-range LOH and whole chromosome LOH were detected in evolved strains. By contrast, using rapid adaptive evolution, aneuploidy was detected in the adaptive strains. It was further found that deletion of gene GLN3, long-range LOH in the left arm of synthetic chromosome X, whole chromosome LOH of synthetic chromosome X, and duplication of chromosome VIII (trisomy) lead to increased rapamycin resistance in synthetic yeast. Comparative analysis of genome stability of evolved strains indicates that the aneuploid strain has a higher frequency of degeneration than the SCRaMbLEd strain. These findings enrich our understanding of genetic mechanism of rapamycin resistance in yeast, and provide valuable insights into yeast genome architecture and function.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30900161</PMID><DateCompleted><Year>2020</Year><Month>03</Month><Day>09</Day></DateCompleted><DateRevised><Year>2020</Year><Month>03</Month><Day>09</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1869-1889</ISSN><JournalIssue CitedMedium="Internet"><Volume>62</Volume><Issue>3</Issue><PubDate><Year>2019</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Science China. Life sciences</Title><ISOAbbreviation>Sci China Life Sci</ISOAbbreviation></Journal><ArticleTitle>Loss of heterozygosity by SCRaMbLEing.</ArticleTitle><Pagination><MedlinePgn>381-393</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s11427-019-9504-5</ELocationID><Abstract><AbstractText>Genetic variation drives phenotypic evolution within populations. Genetic variation can be divided into different forms according to the size of genomic changes. However, study of large-scale genomic variation such as structural variation and aneuploidy is still limited and mainly based on the static, predetermined feature of individual genomes. Here, using SCRaMbLE, different levels of loss of heterozygosity (LOH) events including short-range LOH, long-range LOH and whole chromosome LOH were detected in evolved strains. By contrast, using rapid adaptive evolution, aneuploidy was detected in the adaptive strains. It was further found that deletion of gene GLN3, long-range LOH in the left arm of synthetic chromosome X, whole chromosome LOH of synthetic chromosome X, and duplication of chromosome VIII (trisomy) lead to increased rapamycin resistance in synthetic yeast. Comparative analysis of genome stability of evolved strains indicates that the aneuploid strain has a higher frequency of degeneration than the SCRaMbLEd strain. These findings enrich our understanding of genetic mechanism of rapamycin resistance in yeast, and provide valuable insights into yeast genome architecture and function.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Yunxiang</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, 300072, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Yi</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, 300072, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ma</LastName><ForeName>Lu</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, 300072, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Guo</LastName><ForeName>Zhou</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, 300072, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xiao</LastName><ForeName>Wenhai</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, 300072, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yuan</LastName><ForeName>Yingjin</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, 300072, China. yjyuan@tju.edu.cn.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China. yjyuan@tju.edu.cn.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>03</Month><Day>01</Day></ArticleDate></Article><MedlineJournalInfo><Country>China</Country><MedlineTA>Sci China Life Sci</MedlineTA><NlmUniqueID>101529880</NlmUniqueID><ISSNLinking>1674-7305</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C071664">GLN3 protein, S cerevisiae</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D029701">Saccharomyces cerevisiae Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014157">Transcription Factors</NameOfSubstance></Chemical><Chemical><RegistryNumber>W36ZG6FT64</RegistryNumber><NameOfSubstance UI="D020123">Sirolimus</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="CommentIn"><RefSource>Sci China Life Sci. 2019 Jun;62(6):868-869</RefSource><PMID Version="1">30927172</PMID></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName UI="D000782" MajorTopicYN="Y">Aneuploidy</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015825" MajorTopicYN="N">Chromosomes, Fungal</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004352" MajorTopicYN="N">Drug Resistance, Microbial</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019143" MajorTopicYN="N">Evolution, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005821" MajorTopicYN="Y">Genetic Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014644" MajorTopicYN="N">Genetic Variation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016681" MajorTopicYN="N">Genome, Fungal</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D042822" MajorTopicYN="N">Genomic Instability</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006579" MajorTopicYN="N">Heterozygote</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019656" MajorTopicYN="N">Loss of Heterozygosity</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010641" MajorTopicYN="N">Phenotype</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012441" MajorTopicYN="N">Saccharomyces cerevisiae</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D029701" MajorTopicYN="N">Saccharomyces cerevisiae Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020123" MajorTopicYN="N">Sirolimus</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014157" MajorTopicYN="N">Transcription Factors</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">SCRaMbLE</Keyword><Keyword MajorTopicYN="N">aneuploidy</Keyword><Keyword MajorTopicYN="N">loss of heterozygosity (LOH)</Keyword><Keyword MajorTopicYN="N">rapid adaptive evolution</Keyword><Keyword MajorTopicYN="N">structural variation</Keyword><Keyword MajorTopicYN="N">synthetic yeast genome</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>01</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>02</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>3</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>3</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>3</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30900161</ArticleId><ArticleId IdType="doi">10.1007/s11427-019-9504-5</ArticleId><ArticleId IdType="pii">10.1007/s11427-019-9504-5</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Li, Yunxiang" sort="Li, Yunxiang" uniqKey="Li Y" first="Yunxiang" last="Li">Yunxiang Li</name></noRegion><name sortKey="Guo, Zhou" sort="Guo, Zhou" uniqKey="Guo Z" first="Zhou" last="Guo">Zhou Guo</name><name sortKey="Guo, Zhou" sort="Guo, Zhou" uniqKey="Guo Z" first="Zhou" last="Guo">Zhou Guo</name><name sortKey="Li, Yunxiang" sort="Li, Yunxiang" uniqKey="Li Y" first="Yunxiang" last="Li">Yunxiang Li</name><name sortKey="Ma, Lu" sort="Ma, Lu" uniqKey="Ma L" first="Lu" last="Ma">Lu Ma</name><name sortKey="Ma, Lu" sort="Ma, Lu" uniqKey="Ma L" first="Lu" last="Ma">Lu Ma</name><name sortKey="Wu, Yi" sort="Wu, Yi" uniqKey="Wu Y" first="Yi" last="Wu">Yi Wu</name><name sortKey="Wu, Yi" sort="Wu, Yi" uniqKey="Wu Y" first="Yi" last="Wu">Yi Wu</name><name sortKey="Xiao, Wenhai" sort="Xiao, Wenhai" uniqKey="Xiao W" first="Wenhai" last="Xiao">Wenhai Xiao</name><name sortKey="Xiao, Wenhai" sort="Xiao, Wenhai" uniqKey="Xiao W" first="Wenhai" last="Xiao">Wenhai Xiao</name><name sortKey="Yuan, Yingjin" sort="Yuan, Yingjin" uniqKey="Yuan Y" first="Yingjin" last="Yuan">Yingjin Yuan</name><name sortKey="Yuan, Yingjin" sort="Yuan, Yingjin" uniqKey="Yuan Y" first="Yingjin" last="Yuan">Yingjin Yuan</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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